Detection of Wnt-9a with MAB3157. Recombinant protein and lysates prepared in reducing sample buffer at 5 x 106 cells/mL were resolved by SDS-PAGE and transferred to an Immobilon-P membrane. The blot was developed with 5 μg/mL MAB3157 with chemiluminescent detection. Lane 1: 20 ng rhWnt-9a; Lane 2: 10 μL KATO-III (human gastric carcinoma) cell lysate; Lane 3: 10 μL ME-180 (human cervical carcinoma) cell lysate; Lane 4: 10 μL T47-D (human breast cancer) cell lysate.